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    Lecturer Dr Stefano Pluchino is pleased to consider applications from prospective Ph D students.Department of Clinical Neurosciences Cambridge Centre for Brain Repair and MRC Cambridge Stem Cell Institute [email protected]

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    Join us on Labor Day Weekend to celebrate, well, whatever you want - the start of the school year, the end of summer, the coming of fall (and winter! There will be over 22 varieties of craft beer to sample and 3 live bands occupying Stage Wisp.

    Confocal microscopy image of two intravenously-injected β-gal NPCs (green) persisting (110 days after cell injection) at the level of a representative CNS perivascular area from a R-EAE mouse in very close contact to CNS-infiltrating encephalitogenic blood-borne CD45 inflammatory cells (red) [Nature 2005; 436: 266-71].

    Click image to view full-size Cell culture Cell transplantation Computational modelling Confocal microscopy Electron microscopy (scanning and transmission) Flow cytometry Fluorescence microscopy h ES cells Immunohistochemistry In situ hybridization In vitro co-cultures Magnetic resonance imaging (MRI) Mathematical modeling Microscopy Non viral delivery technologies (nanoparticles; liposomes) Reverse engineering RNA deep sequencing Semi-quantitative real time RT-PCR si RNA Unbiased stereology Viral vector technology for cell engineering and nc RNA delivery Cusimano M, Biziato D, Brambilla E, Donegà M, Alfaro-Cervello C, Snider S, Salani G, Pucci F, Comi G, Garcia-Verdugo JM, De Palma M, Martino G, Pluchino S (2012), “Transplanted neural stem/precursor cells instruct phagocytes and reduce secondary tissue damage in the injured spinal cord.” Details Bacigaluppi M, Pluchino S, Peruzzotti Jametti L, Kilic E, Kilic U, Salani G, Brambilla E, West MJ, Comi G, Martino G, Hermann DM (2009), “Delayed post-ischaemic neuroprotection following systemic neural stem cell transplantation involves multiple mechanisms.” 132(Pt 8):2239-51 Details Pluchino S, Gritti A, Blezer E, Amadio S, Brambilla E, Borsellino G, Cossetti C, Del Carro U, Comi G, 't Hart B, Vescovi A, Martino G (2009), “Human neural stem cells ameliorate autoimmune encephalomyelitis in non-human primates.” 66(3):343-54 Details Pluchino S, Zanotti L, Brambilla E, Rovere-Querini P, Capobianco A, Alfaro-Cervello C, Salani G, Cossetti C, Borsellino G, Battistini L, Ponzoni M, Doglioni C, Garcia-Verdugo JM, Comi G, Manfredi AA, Martino G (2009), “Immune regulatory neural stem/precursor cells protect from central nervous system autoimmunity by restraining dendritic cell function.” 4(6):e5959 Details Pluchino S, Muzio L, Imitola J, Deleidi M, Alfaro-Cervello C, Salani G, Porcheri C, Brambilla E, Cavasinni F, Bergamaschi A, Garcia-Verdugo JM, Comi G, Khoury SJ, Martino G (2008), “Persistent inflammation alters the function of the endogenous brain stem cell compartment.” 131(Pt 10):2564-78 Details Pluchino S, Zanotti L, Rossi B, Brambilla E, Ottoboni L, Salani G, Martinello M, Cattalini A, Bergami A, Furlan R, Comi G, Constantin G, Martino G (2005), “Neurosphere-derived multipotent precursors promote neuroprotection by an immunomodulatory mechanism.” 436(7048):266-71 Details Pluchino S, Quattrini A, Brambilla E, Gritti A, Salani G, Dina G, Galli R, Del Carro U, Amadio S, Bergami A, Furlan R, Comi G, Vescovi AL, Martino G.

    The distribution pattern of L1 might provide a basis for understanding its roles in LTP, fear conditioning, and other unknown functions in the brain.

    For this reason, we studied the total distribution of L1 in the adult mouse CNS using specific polyclonal antisera against full-length L1 and the C-terminal cytoplasmic domain of L1 at the light microscopic level.

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